BJC has received a speaking honorarium from Amgen Inc. Dr fimbriae operon of uropathogenic Escherichia coli mediate microtubule-dependent invasion to the HeLa epithelial cell-line. Escherichia coli DraE adhesin-associated bacterial internalization by epithelial cells is promoted independently by decay-accelerating factor and carcinoembryonic antigen-related cell adhesion molecule binding and does not require the DraD invasin. Clinical trial: randomised placebo-controlled study of clarithromycin in active Crohn's disease. Opposite effects on human colon cancer cell proliferation of two dietary Thomsen-Friedenreich antigen-binding lectins. Characterization, of epithelial IL-8 response to inflammatory bowel disease mucosal E-coli and its inhibition by mesalamine. Clinical Science, 4 Lectin methods and protocols. Following the induction of high copy number of fosmids during bacterial growth Epicentre copy-control auto-induction solutionclones from haemagglutination-positive E coli were extracted using Qiafilter midi-kits Qiagen; Crawley, UK.
Jon Rhodes University of Liverpool
Additional article information . Increased prevalence in CD and CRC of DAEC possessing lpfA and fimH relevant to M cell translocation. Article (PDF Available) in Gut 63(5) · July with 90 Reads E coli expressing both afaC and lpfA (relevant to M-cell translocation) were common in CD (8/14, p=) and CRC (14/21, p=), but not. of Liverpool, Liverpool L Inﬂammatory bowel disease ORIGINAL ARTICLE Colonic mucosa-associated diffusely adherent afaC+ Escherichia coli expressing lpfA and pks are increased .
High prevalence of adherent-invasive Escherichia coli associated with ileal mucosa in Crohn's disease.
Molecular characterization of a fimbrial adhesin, F, mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp-2 cells. Dr fimbriae operon of uropathogenic Escherichia coli mediate microtubule-dependent invasion to the HeLa epithelial cell-line. The Journal of biological chemistry, 27 Significance of this study.
Peanut agglutinin appearance in the blood circulation after peanut ingestion mimics the action of endogenous galectin-3 to promote metastasis by interaction with cancer-associated MUC1 Journal article Zhao, Q.
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|Increased C adherence to, and D invasion of differentiated Caco2 cells by afa-1 -possessing clones was observed.
Functional adhesin was confirmed by haemagglutination and adhesion to HEp-2 cells see supplementary file S2, available online only. London: Mosby-Wolfe. Interaction between bacterial peptides, neutrophils and goblet cells: a possible mechanism for neutrophil recruitment and goblet cell depletion in colitis.
Randomised placebo-controlled trial of rituximab anti-CD20 in active ulcerative colitis Journal article Leiper, K. Irving Ed. Translocation through M cells in culture Translocation through M cells, generated by co-culture of Caco2-cl1 and Raji-B cells, was conducted as previously described.
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Escherichia colihost macrophage interactions in the pathogenesis of inflammatory bowel disease
Replication of Crohn's Disease Mucosal E. coli Isolates inside of Translational Medicine, University of Liverpool, Liverpool L69 3GE, UK. 2 diffusely adherent afaC+ Escherichia coli expressing lpfA and pks are. Total Article Views () lpfA: Gene encoding long polar fimbriae adhesin; M cells: Microfold cells; ompC: Gene encoding outer-membrane.
It has been shown previously that DAEC are partly resistant to phagocytosis by neutrophils.
Journal article Agrawal, A.
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The Journal of biological chemistry, 1 Gastroenterology ; —93 [ PubMed ] [ Google Scholar ]. Generation of an afa - 1 -expressing recombinant E coli Following sequence identification of Afa-1 as the putative adhesin responsible for haemagglutination, purified fosmid was extracted from haemagglutination-positive clone E coli 8H8.
Significance of this study.
Richman E, Rhodes JM: Review article: evidence-based dietary. Article · Figures · Info & Metrics · PDF Institute of Translational Medicine, University of Liverpool, Duncan Building, Daulby Street, Liverpool L69 3GA, U.K.
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Articles published by this Open-Access journal are distributed under the of Translational Medicine, University of Liverpool, Liverpool L69 3GE, United Kingdom lpfA and pks are increased in inflammatory bowel disease and colon cancer.
Siegel, D. Biochemical Society transactions, 36 Pt 6 DOI: The presence of the afa-1 operon in haemagglutinating E coli correlates with diffuse adherence and invasion to HEp-2 epithelial cells. Mucosal glycoconjugates in inflammatory bowel disease and colon cancer.
Haemagglutinin-positive E coli in the fosmid clone library derived from E coli HM share a common region containing the afimbrial adhesin afa-1 operon.
These properties have led to them being designated AIEC.
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|Intestinal inflammation targets cancer-inducing activity of the microbiota.
Suppression of core 1 Gal-transferase is associated with reduction of TF and reciprocal increase of Tn, sialyl-Tn and Core 3 glycans in human colon cancer cells. Published online Jul Rhodes, J.
We can confirm that to date all requests from researchers within the international scientific community for isolates from the Liverpool archive have been granted and materials supplied. Trends in Molecular Medicine. Impact of specialist care on clinical outcomes for medical emergencies.